Application

Research CategoryCell Structure

Dot Blot Analysis: A representative lot detected Platelet in Dot Blot applications (Shattil, S.J., et. al. (1985). J Biol Chem. 260(20):11107-14).

ELISA Analysis: A representative lot detected Platelet in ELISA applications (Shattil, S.J., et. al. (1985). J Biol Chem. 260(20):11107-14).

Radioimmunoassay Analysis: A representative lot detected Platelet in Radioimmunoassy applications (Shattil, S.J., et. al. (1985). J Biol Chem. 260(20):11107-14; Shattil, S.J., et. al. (1987). Blood. 70(1):307-15).

Western Blotting Analysis: A representative lot detected Platelet in Western Blotting applications (Shattil, S.J., et. al. (1985). J Biol Chem. 260(20):11107-14).

Flow Cytometry Analysis: A representative lot detected Platelet in Flow Cytometry applications (Caron, A., et. al. (2002). J Cardiovasc Pharmacol. 40(2):296-306; Taub, R., et. al. (1989). J Biol Chem. 264(1):259-65; Shattil, S.J., et. al. (1987). Blood. 70(1):307-15).

Flow Cytometry Analysis: A 1:200 dilution from a representative lot detected Platelet in freshly isolated human platelets activated with convulxin (Courtesy of Shuchi Gupta, PhD, University of Pennsylvania, USA).

Anti-Platelet, clone PAC-1, Cat. No. MABT893 is a mouse monoclonal antibody that detects Integrin alpha-IIb and has been tested for use in Dot Blot, ELISA, Flow Cytometry, Radioimmunoassay, and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Integrin alpha-IIb (UniProt: P08514; also known as GPalpha IIb, GPIIb, Platelet membrane glycoprotein IIb, CD41) is encoded by the ITGA2B (also known as GP2B, ITGAB) gene (Gene ID: 3674) in human. GPIIb is a single-pass type I membrane protein of the integrin alpha chain family. It serves as a receptor of fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. . Following activation it brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation, which physically plugs ruptured endothelial cell surface. GPIIb is synthesized with a signal peptide of 31 amino acids that is cleaved to produce the mature form that has an extracellular domain (aa 32-993), a helical domain (aa 994-1019), and a cytoplasmic tail (aa 1020-1039). Three isoforms of GPII b are reported that are generated by alternative splicing. Isoform 1 and 2 have been identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U-937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells. Clone PAC- 1 is a pentameric IgM that is reported to bind to the activated platelets, but not to unstimulated platelets.

Immunogen

Platelets obtained from the blood of a patient with Glanzmann′s thrombasthenia.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified by ion-exchange chromatography

Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.

Format: Purified

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgM.

Specificity

Clone PAC-1 is a pentameric IgM that binds to the activated platelets, but not to unstimulated platelets.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

113.38 kDa calculated.